Alexa Fluor®系列荧光染料激发和发射波谱汇总资料

2020/05/14 admin 185

Alexa Fluor®系列荧光染料激发和发射波谱汇总资料

Alexa Fluor®是Molecular Probes(现已属于Thermo Fisher)的注册商标。Alexa系列染料的取名自分子探针公司创始人之一Dick Haugland的儿子Alex Haugland。

华泰昕微粒

Table 1. Properties of Alexa Fluor Dyes

Alexa Fluor dyeAbsorption
max. (nm)
Emission
max (nm)
Emission color*Extinction
coefficient**
Alexa Fluor 350346442Blue19,000
Alexa Fluor 405401421Blue34,000
Alexa Fluor 430433541Green/Yellow16,000
Alexa Fluor 488496519Green71,000
Alexa Fluor 532532553Yellow81,000
Alexa Fluor 546556573Orange104,000
Alexa Fluor 555555565Orange150,000
Alexa Fluor 568578603Orange/Red91,000
Alexa Fluor 594590617Red73,000
Alexa Fluor 610612628Red138,000
Alexa Fluor 633632647Far Red239,000
Alexa Fluor 635633647Far Red140,000
Alexa Fluor 647650665Near-IR***239,000
Alexa Fluor 660663690Near-IR***132,000
Alexa Fluor 680679702Near-IR***184,000
Alexa Fluor 700702723Near-IR***192,000
Alexa Fluor 750749775Near-IR***240,000
Alexa Fluor 790784814Near-IR***270,000
* Typical emission color seen through the eyepiece of a conventional fluorescence microscope with appropriate filters.
** Extinction coefficient at lmax in cm–1M–1. 
*** Human vision is insensitive to light beyond ~650 nm; it is not possible to view near-IR fluorescent dyes.

Table 2. Common fluorescence excitation sources

SourcePrincipal lines (nm)
Mercury arc lamp366, 405, 436, 546, 578*
Xenon arc lamp250-1000*
Tungsten–halogen lamp350-1000*
Blue diode laser405
Helium–cadmium laser325, 442
Argon ion laser457, 488, 514
Nd:YAG laser532**
Helium–neon laser543, 594, 633
Yellow diode laser561
Krypton ion laser568, 647
Red diode laser635
* Continuous white-light source.
** Frequency-doubled principal line output.

Table 3. Alexa Fluor Dye equivalents of common dyes

If you are usingTry this Alexa Fluor dye
AMCA, coumarinAlexa Fluor 350
Cy2, FITC (fluorescein)Alexa Fluor 488
Cy3, TRITC (rhodamine)Alexa Fluor 555
Rhodamine redAlexa Fluor 568
Texas RedAlexa Fluor 594
Cy5Alexa Fluor 647
Cy5.5, IR680Alexa Fluor 680
Cy7Alexa Fluor750


Alexa Fluor®系列荧光染料详情链接(ThermoFisher):

Alexa Fluor 350

Alexa Fluor 405

Alexa Fluor 488

Alexa Fluor 532

Alexa Fluor 546

Alexa Fluor 555

Alexa Fluor 568

Alexa Fluor 594

Alexa Fluor 647

Alexa Fluor 680

Alexa Fluor 750

Pacific Blue

Coumarin

BODIPY FL

Pacific Green

Oregon Green

Fluorescein (FITC)

Cy3

Pacific Orange

Tetramethylrhodamine (TRITC)

Texas Red

Cy5


备注说明:此篇文档仅供查询技术资料用,我公司目前不代理Alexa Fluor®系列产品,如果订购其他如cy或ATTO等系列荧光染料请联系。