Ocean NanoTech's carboxyl terminated gold nanoparticles (GPHs) of various diameters (15 nm-100 nm) can be conjugated with proteins using our easy-to-use conjugation kit (Catalog # GCK). The kit contains all the reagents and a choice of GPHs for 5 conjugation reactions. All buffers and nanoparticles in the kit have been autoclaved.
Briefly, the GPHs are activated with carbodiimide followed by conjugation to amine groups that are present on the proteins. Purification is performed using centrifugation. The protocol shown below has been used to successfully conjugate bovine serum albumin, streptavidin and immunoglobulin to Ocean Nanotech's GPH.