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spheroteach urfp-30-2 超级彩虹荧光微球 3um

超级彩虹荧光微球,UltraRainbow Fluorescent Particles, 1E7/mL, 3.0-3.4 um,URFP-30-2激发波长包括了自UV至远红外的所有通道。彩虹荧光微球的激发波长为365-650 nm,超级彩虹荧光微球即彩虹荧光微球增加紫外通道。New fl ow cytometers, with fl uorescent channels from the UV t

  • 货号: URFP-30-2(spherotech)

超级彩虹荧光微球,UltraRainbow Fluorescent Particles, 1E7/mL, 3.0-3.4 um,URFP-30-2

激发波长包括了自UV至远红外的所有通道。彩虹荧光微球的激发波长为365-650 nm,超级彩虹荧光微球即彩虹荧光微球增加紫外通道。


New fl ow cytometers, with fl uorescent channels from the UV to Far Red, and corresponding fluorescent conjugates are now available. As a result, we have developed the Ultra Rainbow Fluorescent Particles with enhanced UV and Far Red fluorescence intensity. The Ultra Rainbow

Fluorescent Particles contain a single peak and are designed for checking the optical alignment of any flow cytometer in all channels.

Instructions for use:


A. Preparation of Particles
1. Vortex the particles vigorously
2. Add 2 to 4 drops of particles to 1mL of sheath fluid or DI water. The inclusion of a small amount of detergent (~0.01%) in the dilution buffer will increase the percentage of the singlet population.


B. Daily Alignment
To determine the optical alignment of the system perform the following:
1. Set a live gate for the singlet population on the FSC vs SSC histogram to exclude aggregates
2. Adjust the Gain and High voltage so that the mean channel number of the peak is in a predetermined position on each histogram of interest. The histograms on the previous page
can be used as a guide.
3. Collect 5000 events inside the gate
4. Record the % CV and High Voltage for all fluorescence channels of interest.
5. Use a computer program such as Excel to generate the Levy Jennings graphs.


NOTE: If the values on any parameter exceed those of the day-to-day average or preset values, which are determined by at least one months worth of data, additional calibration or alignment procedures should be performed according to the instrument operation manual.


订购链接:Spherotech 超级彩虹荧光微球


品牌授权代理商,订购或询价:北京华泰昕生物医疗技术有限公司

电话:010-83131025

email: info@hypercyte.com

QQ: 2428 012 469

标签: URFP-30-2