背景资料:
This antibody preparation contains a mixture of purified rat monoclonal antibodies
directed against mouse GPIb (CD42b). Targeting of this receptor with divalent IgGs results in
profound and irreversible Fc-independent platelet depletion in mice1,2
. Platelet depletion is frequently
used to study the general role of platelets in (patho-) physiology3
.
保存条件:
The antibodies were purified from hybridoma cell culture supernatant by
Protein G-Sepharose chromatography.
Stable for six months from date of shipment when stored at
4°C.
KEEP STERILE, the preparation contains no preservative. Aliquots can be stored at -20°C for at
least one year. Avoid repeated freezing and thawing.
应用:
This preparation is optimized to reduce the platelet count by >95% within 60 minutes after
intravenous injection of 2 µg/g bodyweight. It is recommended to inject the antibodies in a volume of
100-200 µl (diluted with sterile PBS).
同型对照(抗体来源): Rat IgG (Wistar)
缓冲液: 0.5 mg purified immunoglobulins in PBS, 0.2% (w/v) BSA, sterile filtered
浓度: 0.5 mg/ml