Bangs Laboratories

Quantum™ 和 Quantum™ Simply Cellular® (QSC) kits

2020/07/24 admin 47

Quantum™ MESF and Quantum™ Simply Cellular® beads are external standards that enable the standardization of fluorescence intensity units irrespective of cytometer and software. Because they are labeled with the same fluorochromes used to label cells, they provide a  synchronous response to the environment. To use, beads are run on the same day and at the same PMT & compensation settings as samples to establish a calibration curve that relates instrument channel values and standardized fluorescence intensity units (MESF or ABC). Cell samples are then read against the curve for determination of expression (i.e. quantitation of the fluor signal from each cell population).


QUANTUM™ MESF

Our Quantum™ MESF kits contain one blank population and a series of either four fluorescent microsphere populations labeled with varying amounts of Alexa Fluor® 488, Alexa Fluor®647, APC, Cy™5, PE-Cy™5, R-PE or Pacific Blue™, or five fluorescent microsphere populations labeled with varying amounts of FITC. The assignment of fluorescence intensity in Molecules of Soluble Fluorochrome (MESF) units is performed through direct comparison of fluorescence measurements from solutions of the pure fluorochrome with those from microspheres surface-labeled with the same fluorochrome. See PDS 849 to determine effective FP ratio. A free QuickCal® analysis template is provided with each kit to aid in determining the expression level of cells, and for evaluating instrument linearity and detection threshold. Quantum™ MESF kits are available in three sizes: A (20 tests), B (100 tests), and C (280 tests).

Notice to FITC-5 Customers: In our continual efforts to manufacture the most stable FITC MESF kits, we are utilizing an enhanced calibration strategy that features additional data points to best stabilize assignments through time. Though we anticipate that changes will not be evident for most customers, some may observe a slight shift in the calibration curve, observable in low or high assignments.

RELATED PRODUCTS

目录册页 PDS821

技术手册

Cat. Code Description Sizes 

488 Quantum™ Alexa Fluor® 488 MESF 1mL, 5mL, or 14mL 

647 Quantum™ Alexa Fluor® 647 MESF 1mL, 5mL, or 14mL 

823 Quantum™ APC MESF 1mL, 5mL, or 14mL 

822 Quantum™ Cy™5 MESF 1mL, 5mL, or 14mL 

555 Quantum™ FITC-5 MESF 1mL, 5mL, or 14mL 

555p Quantum™ FITC-5 MESF (Premix) 1mL, 5mL, or 14mL 

828 Quantum™ PE-Cy™5 MESF 1mL, 5mL, or 14mL 

827 Quantum™ R-PE MESF 1mL, 5mL, or 14mL


QUANTUM™ SIMPLY CELLULAR®

Quantum™ Simply Cellular® (QSC) kits are comprised of five microsphere populations that are ~7-9µm in diameter: one blank and four labeled with increasing amounts of antibody. Each coated population binds a specific number of monoclonal antibodies of the noted species, which is equal to its Antibody Binding Capacity (ABC) value. The investigator labels QSC beads using the same antibodies used to stain cells.  A free QuickCal® analysis template is provided with each kit to aid in determining the expression level of cells, and for evaluating instrument linearity and detection threshold. Quantum™ Simply Cellular® kits are available in three sizes: A (20 tests), B (100 tests), and C (280 tests). For additional information about working with these products, see our product data sheet on Antibody Titration or check out PDS 818, Tips and Troubleshooting.

QUANTUM™ SIMPLY CELLULAR®产品订购信息

产品目录PDS814

Cat. Number Description Sizes 

815 Quantum™ Simply Cellular® anti-Mouse IgG 1mL, 5mL, or 14mL 

816 Quantum™ Simply Cellular® anti-Human IgG 1mL, 5mL, or 14mL 

817 Quantum™ Simply Cellular® anti-Rat IgG 1mL, 5mL, or 14mL


SIMPLY CELLULAR®

Our Simply Cellular® standard consists of a single population of antibody-coated microspheres (~7-9µm in diameter) of known Antibody Binding Capacity (ABC). The population is stained with the user's primary antibody and used in conjunction with a suitable Quantum™ MESF kit for determination of the antibody's effective Fluorophore : Protein (F/P) ratio. This standard may also be used to QC the fluorescence intensity of different antibody lots or clones. Simply Cellular® kits are available in three sizes: A (20 tests), B (100 tests), and C (280 tests). For additional information about working with these products, see our product data sheet on Antibody Titration or check out PDS 818, Tips and Troubleshooting. 

SIMPLY CELLULAR®产品订购信息货号和描述

PDS810产品目录

Cat. Number Description Sizes 

810 Simply Cellular® anti-Mouse IgG 1ml, 5mL, or 14mL 

812 Simply Cellular® anti-Human IgG 1ml, 5mL, or 14mL 

813 Simply Cellular® anti-Rat IgG 1ml, 5mL, or 14mL