关于同型对照(Isotype Control)

2014/10/25 admin

同型对照(Isotype Control):使用与一抗相同种属来源、相同亚型、相同剂量和相同的免疫球蛋白及亚型的免疫球蛋白,用于检测由于抗体非特异性结合到组织细胞而产生的背景染色。

如果一抗是多抗,可以用normal serum(与一抗相同的正常血清) ;如果一抗是单抗,如mouse anti rat CD11b,clone OX-42 的 Isotype 是 mouse IgG2a, 可以用purified(纯化的) mouse IgG2a来做 OX-42的同型对照(Isotype Control)。


Isotype controls are used to confirm that the primary antibody binding is specific and not a results of non-specific Fc receptor binding or other protein interactions. The isotype control antibody should match the primary antibody’s host species, isotype, and conjugation. For example, if the primary antibody is a FITC-conjugated mouse IgG1, then you will need to choose a FITC-conjugated mouse IgG1 isotype control.


To find a suitable isotype control on our website, go to the Products section of the top navigation menu. Choose ";isotype control";, then the species and subclass of your primary antibody. This will give you a range of isotype controls for use with that species and isotype of primary antibody.


Isotype controls for polyclonals:
Most isotype controls are monoclonal. They are not suitable for use with polyclonal antibodies as these contain more than one IgG subclass. However, we do provide several polyclonal isotype controls for use in these circumstances.