氨基磁珠微球结合能力的测定

2014/10/25 admin 73

MATERIALS:
1. Avidin magnetic particles, 1% w/v, Cat. # VM-40-10, Lot No. J01, 4.35 μm
2. Biotin-FA conjugate, Lot No. 021688, 533 nM in 1% diluent. (IBS containing 1% normal goat serum and 1% fetal bovine serum )

 

PROCEDURES:
1. Add 50, 100,150, 200, 300 & 400 μl of Avidin magnetic particles to six 12x75 test tubes.
2. Add 1 mL of Biotin-FA conjugate to each tube.
3. Vortex and incubate at ambient temperature for at least 30 minutes with occasional shaking.
4. Adjust the fluorimeter for excitation and emission at 490 and 520 nm respectively

 

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