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流式细胞仪阈值设定微球,Flow Cytometry Particles For Threshold Determination

2015/03/24 华泰昕生物|HyperCyte 已读
The SPHERO™ Blank Calibration Particles are designed for the determination of the flow cytometer's fluorescence threshold. It is important to determine if the autofluorescence level of a sample is above the fluorescence threshold of the instrument. To measure the fluorescence threshold of the instrument run the Blank Calibration Particles at the same instrument settings and determine if they are less fluorescent than the sample. The fluorescence threshold of the instrument can be quantitatively determined by calibrating the instrument using the same instrument settings with calibrated standards such as the Rainbow or Ultra Rainbow Calibration Particles.

 

SPHERO™ Blank Calibration Particles
Catalog No.
Description
Concentration
Nominal Size
Package Size
BCP-10-5
Blank Calibration Particles
107/mL
1.0-1.4 µm
5 mL
BCP-20-5
Blank Calibration Particles
107/mL
1.8-2.2 µm
5 mL
BCP-30-5
Blank Calibration Particles
107/mL
3.2 (+/-0.1 µm)
5 mL
BCP-32-5
Blank Calibration Particles
107/mL
3.0-3.4 µm
5 mL
BCP-35-5
Blank Calibration Particles
107/mL
3.5-4.0 µm
5 mL
BCP-50-5
Blank Calibration Particles
107/mL
5.0-5.9 µm
5 mL
BCP-60-5
Blank Calibration Particles
107/mL
6.0-6.4 µm
5 mL
BCP-70-5
Blank Calibration Particles
107/mL
6.5-8.0 µm
5 mL
BCP-100-5
Blank Calibration Particles
5x106/mL
8.0-12.9 µm
5 mL
BCP-150-5
Blank Calibration Particles
5x106/mL
13.0-17.9 µm
5 mL
BCP-200-5
Blank Calibration Particles
0.5x106/mL
18.0-24.9 µm
5 mL
BCP-300-5
Blank Calibration Particles
0.5x106/mL
25.0-35.0 µm
5 mL

 




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