GenePORTER® 2 Transfection Reagent is a novel lipid formulation specifically designed to provide high transfection efficiencies in a broad range of mammalian cells. GenePORTER 2 Reagent is especially effective in difficult-to-transfect cells, such as PC-12, macrophages, and primary cells. Developed using a high-throughput transfection assay that screens a large library of cationic lipids, the GenePORTER 2 Reagent was selected based on high transfection efficiency, low cytotoxicity, and exceptional stability. Additionally, because of its minimized interaction with serum components, the GenePORTER 2 Reagent is ideal for transfection in serum containing media.
|成分 \ 货号||Cat. No. T202007||Cat. No. T202015||Cat. No. T202075||Cat. No. T202096|
|GenePORTER 2 Transfection Reagent||1 vial of dried lipid film||1 vial of dried lipid film||5 vials of dried lipid film||96 vials of dried lipid film|
|Hydration Buffer||1 vial x 0.75 ml||1 vial x 1.5 ml||5 vials x 1.5 ml||None|
|DNA Diluent||2 vials x 2 ml||4 vials x 2 ml||20 vials x 2 ml||1 vial x 6 ml|
|DNA Diluent B||1 vial x 4 ml||1 vials x 8 ml||5 vials x 8 ml||1 vial x 8 ml|
The GenePORTER 2 Transfection Reagent is shipped at room temperature. For maximum stability, store all reagents at 4C upon receipt. If stored properly, the dried GenePORTER 2 reagent is stable for 12 months, and the hydrated GenePORTER 2 reagent is stable for 6 months. The Hydration Buffer and DNA Diluent are stable for 12 months.
75 rxn kit, 0.75 ml, T202007
150 rxn kit, 1.5 ml, T202015
750 rxn kit, 5 x 1.5 ml, T202075
QuikEase kit, 96 single-use tubes, T202096
DNA Diluent, 5 x 8 ml, T20DA05
DNA Diluent, 10 x 8 ml, T20DA10
DNA Diluent B, 5 x 8 ml, T20DB05
DNA Diluent B, 10 x 8 ml, T20DB10
GenePORTER 2 Cell Line Citations: 3T3-L1, A549, AD293, alpha-T3, B103, BCBL-1, BV2, C2C12,Caco-2, CHO, CHO CHO-K1, CHO-K1, CL3, COLO 320DM, COS-1, COS-7, GH4C1, GP202, H19-7, HaCaT, HEK 293, HEK 293 HEK 293T HEK EBNA, HEK-293T, HeLa, HEp-2, Hep3B, HepG2, HT 1080, HUVEC, IB3.1, JEG3, K562, KB, KNRK, L929, LbetaT2, MCF-7, MDBK, MDCK, MEF, MEF-3T3, NC-37, NCI-H23, NIH-3T3, P19, PC-12, PC-3, Phi-NX, PLC ,Primary Retinal Pericytes, Primary Dermal Cells, Primary HPAEC, Primary Pulmonary Artery Endothelium, Primary Osteoblasts, Primary Astrocytes, Primary Keratinocyte, Primary Fetal Kidney, Primary Dermal Fibroblast, Primary Keratinocytes, Primary PASMC, PtK2, Rat-1, RAW 264.7, RAW264.7, RBL-2H3, Saos-2, SH-SY5Y, SiHa, SNB19, SW13, SW1353, TKPTS, Φ NX, Phi NX.
US Patent 6,348,499
High transfection efficiencies in difficult-to-transfect cells
Compatibility with serum-containing media