EUROFLOW

The EuroFlow Consortium consists of 20 diagnostic research groups and one associated SME, which are regarded as experts in the fields of flow cytometric and molecular diagnostics.

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The EuroFlow Consortium consists of 20 diagnostic research groups and one associated SME, which are regarded as experts in the fields of flow cytometric and molecular diagnostics. Together the 20 participants have sufficient complementarity and congruence to cover all aspects of development, standardization, and validation of highly sensitive tests for diagnosis and follow-up.

The main objective of the EuroFlow Consortium is the development and standardization of fast, accurate, and highly sensitive flow cytometric tests for diagnosis and prognostic (sub)classification of hematological malignancies as well as for evaluation of treatment effectiveness during follow-up. This includes the introduction of novel flow cytometric methods for replacing several types of molecular techniques (particularly PCR-based detection of fusion genes and oncogenes).

EuroFlow is a division of ESLHO (European Scientific foundation for Laboratory HematoOncology). Other divisions of ESLHO are EuroClonality (www.euroclonality.org) and EuroMRD (www.euromrd.org). General information about ESLHO can be found on the ESLHO website (www.eslho.org)

ESLHO is an official EHA Scientific Working Group (SWG). SWGs seek to initiate and create scientific networks that serve under the umbrella of EHA. At the annual EHA Congress, ESLHO presents its innovations in the special EHA-SWG sessions.


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校准微球 SPHERO™ Rainbow calibration particles

The Rainbow calibration particles, 8 peaks are designed for routine calibration of flow cytometers. Rainbow calibration particles, 8 peaks are commonly used in many laboratories for Quality Control and long-term performance of the analyser.

Rainbow calibration particles, 8 peaks contain several similar sized particles that are dyed in eight different fluorescent intensities.  Each particle contains a mixture of fluorophores that are excited at any wavelength from 365 to 650 nm. The same set of particles enables the calibration of all channels in the flow cytometer. The Rainbow Particles have emission spectra compatible with many common fluorochromes used for immunofluorescent staining.

Rainbow calibration particles, 8 peaks (Rainbow calibration, 8 peaks Cat. No. RCP-30-5A) are used in EuroFlow™ standardization guidelines throughout the study of initial PMT characterization, to set target MFI values and daily performance tracking of the flow cytometers.

订购货号:18-RCP-35-5A(EUROFLOW)

规格:5mL

 

  • EuroFlow™ reference target values (7th peak) for FACSCanto™ II

        Batch EAB01, EAC01, EAD05, EAE01, EAF01, EAG01 (check values)

 


Lower MFI (-15%)

Rainbow 7-peak (DiVa)

Upper MFI (+15%)

Pacific Blue™

100.452

118.178

135.905

Pacific Orange

93.871

110.436

127.002

FITC

28.752

33.826

38.900

PE

32.381

38.095

43.809

PerCP

66.846

78.642

90.438

PC7

8.316

9.783

11.250

APC

158.639

186.634

214.629

APC-H7

64.194

75.522

86.850

FACSCanto™ is a trademark of Becton, Dickinson and Company. Pacific Blue™ and Pacific Orange™ are trademarks of Life Technologies Corporation. Cy™ is a trademark of GE Healthcare.
EuroFlow™ is a trademark of European Scientific foundation for Laboratory Hemato Oncology (ESLHO).


  • EuroFlow™ reference target values (7th peak) for Navios™ or Gallios™

        Batch EAB01, EAC01, EAD05, EAE01, EAF01; EAG01 (check values)

 


Lower MFI (-15%)

Rainbow 7-peak (Navios)

Upper MFI (+15%)

FL9 – Pacific Blue™

379,874

446,911

513,948

FL10 – OC515

278,720

327,906

377,092

FL1 - FITC

117,719

138,493

159,267

FL2 - PE

101,813

119,780

137,747

FL4 – PerCPCy™5.5

253,075

297,735

342,395

FL5 – PECy™7

22,648

26,645

30,642

FL6 - APC

637,655

750,182

862,709

AFL8 – APC-C750

162,326

190,972

219,618

Navios™ and Gallios™ are trademarks of Beckman Coulter Inc. Pacific Blue™ is a trademark of Life Technologies. Cy™ is a trademark of GE Healthcare.
EuroFlow™ is a trademark of European Scientific foundation for Laboratory Hemato Oncology (ESLHO).

 

EuroFlow™ validated Antibodies

 

NameFormatCatalogue codeSizeCloneIsotypeStatusApplication in EuroFlow™ Panels
CD8FITCCYT-8F8200 testsUCHT-4IgG2aCE/IVDLST,SST,B-CLPD
CD38PURECYT-38P1100 testsLD38IgG1CE/IVDPCD
CD38FITCCYT-38F200 testsLD38IgG1CE/IVDPCD
CD45OC515™CYT-45OC100 testsGA90IgG2aCE/IVDALOT, LST, SST, AML, B-CLPD, BCP-ALL, NK-CLPD, T-ALL, T-CLPD
CD56PECYT-56PE200 testsC5.9IgG2bCE/IVDLST, SST, PCD, AML, B-CLPD
CyCD79aPECYT-79aPE100 testsHM57IgG1CE/IVDALOT
IgKappaPECYT-KAPPPE100 testsPolyclonalPolyclonalCE/IVDLST, SST, B-CLPD
IgLambdaFITCCYT-LAMBF100 testsPolyclonalPolyclonalCE/IVDLST, SST, B-CLPD
IgLambdaAPC-C750CYT-LAC75050 testsPolyclonalPolyclonalCE/IVDPCD, BCP-ALL
CyMPOFITCCYT-MPOF3100 tests2C7IgG1RUOALOT
CyTCRβAPCCYT-bF1AP100 tests8A3IgG1RUOT-ALL

 

 

 

EuroFlow™ validated Antibody Cocktails:

 

ReferenceDescriptionFormat
CYT-SLPC-25CD8+Lambda-FITC / CD56+Kappa-PE25 tests
CYT-SLPC4-25CD8+Lambda-FITC / CD56+Kappa-PE / CD4-PerCP-Cy5.525 tests
CYT-SLPC-50CD8+Lambda-FITC / CD56+Kappa-PE50 tests
CYT-SLPC4-50CD8+Lambda-FITC / CD56+Kappa-PE / CD4-PerCP-Cy5.550 tests