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用于时间分辨的羧基修饰铕螯合荧光微粒

2015/03/11 华泰昕生物|HyperCyte 已读

Thermo Scientific™ Fluoro-Max 羧基修饰荧光微粒,通过限制相对短暂的基质荧光背景干扰,降低荧光测定的检测限值。 本产品由染色的 Thermo Scientific™ OptiLink™ 羧基修饰微粒与铕螯合而成,专为基于膜或自动化荧光应用而设计。 Fluoro-Max 铕螯合物微粒具有极宽的斯托克斯切换范围,有助于避免非特异性荧光干扰。

 

微粒是羧基修饰聚苯乙烯微粒内部用铕螯合物染色而成。 这些微粒共有三种标准直径,分别为 0.1、0.2 和 0.3 μm。 这些微粒的激发波长为 333 nm,发射波长为 613 nm,拥有约 0.5 毫秒的超长寿命,是大多数荧光团寿命的约 10,000 到 100,000 倍。 铕螯合物微粒的超长寿命使得它们能够应用于广泛的时间分辨荧光领域。

  • 微粒采用内部染色,可确保最大的色彩亮度和色彩饱和度,从而提供更高的灵敏度和检测结果可读性,同时避免了酶法测定的相关问题。
  • 内部染色防止了在水介质中产生渗滤,并可提供最佳的表面免疫反应性。
  • 染色工艺对免疫球蛋白 G 的吸附或共价耦合存在极少抑制或完全无抑制,从而确保蛋白与微粒的有效结合。
  • 本品应用范围广泛,如临床诊断、免疫/组织学研究等。

推荐用途:

临床诊断、分子生物学和侧向免疫层析、浊度法和玻片凝集法测定

 

华泰昕

Particles are made from carboxylate-modified polystyrene particles containing encapsulated europium chelate dye. They are available in standard 0.1, 0.2 or 0.3μm diameters. When these particles excite at 333nm and emit at 613nm, they do so with a very long lifetime of approximately 0.5 milliseconds, about 10,000 to 100,000 times the lifetime of most fluorophores. The extended lifetime of europium chelate particles allows them to be used in a wide range of time-resolved fluorescence applications.

 

  • Particles are internally dyed to ensure maximum color brilliance and color saturation, resulting in greater sensitivity, enhanced readability of the assay and elimination of problems associated with enzyme-based assays.
  • Encapsulated dye prevents leaching in aqueous media and provides optimum surface immunoreactivity.
  • Dyeing process causes little or no inhibition of adsorption or covalent couping of immunoglobulin G, resulting in superior binding of protein to the particles.
  • Suitable for a variety of applications such as clinical diagnostics, immuno/histological studies and research.

Recommended for:

Clinical diagnostics, molecular biology and lateral flow, turbidimetric and slide aggulation assays

 

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