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苏州医工所血液免疫学研究中心在免疫微球制备方面取得进展

2016/04/21 华泰昕生物|HyperCyte 已读

免疫微球是近年来发展较快的一项免疫学技术。微米级或纳米级的微球由于具有较高的表面积体积比、良好的分散性和易于处理等优点被开发用作抗原或抗体的固体支持物。可溶性的抗原或抗体吸附在微球上形成免疫微球。这些免疫微球可以应用在免疫凝集流式细胞术表面增强拉曼散射等方面。但是人工微球的制备条件较严苛,蛋白结合效率低和成本高等缺点也限制了人工微球的广泛应用。 

近期,苏州医工所血液免疫学研究中心制备并表征了一种红细胞制成的微载体。将羊红细胞用戊二醛处理后可以长期保存,避免溶血,然后在其表面包被上适当比例的磁纳米颗粒,制备成磁化醛化羊红细胞。该方法既能使羊红细胞带有磁性,又不影响羊红细胞表面抗原的暴露。磁化醛化羊红细胞可以在磁场的作用下从溶液中分离出来,避免了离心等繁琐的步骤。 

制备所得的磁化醛化羊红细胞经流式细胞仪检测能与人免疫球蛋白(IgG)有较好的结合。研究结果表明这种磁化醛化羊红细胞可以作为一种微载体应用于流式细胞术。这种方法也可用于其他红细胞微载体的制备。 

相关研究结果已发表在Analytical Letters, 2016, 49(6): 768-777(SCI,IF=1.031)上。 

文章链接:http://www.tandfonline.com/doi/abs/10.1080/00032719.2015.1079207

华泰昕

图1 SEM(扫描电镜)表征的磁化醛化羊红细胞


来源:苏州医工所


原文标题及摘要:

Characterization of a Novel Microcarrier Using Magnetized Red Blood Cells with Terminal Aldehydes

A novel microcarrier based on red blood cells was prepared and characterized. Proof of principle was demonstrated by using sheep red blood cells that were treated with glutaraldehyde to render the cells generally resistant to hemolysis. Magnetic beads of approximately 10 nm in diameter were prepared and attached to the surface of glutaraldehyde-treated sheep red blood cells by electrostatic interaction at various concentrations. The composites were characterized by scanning electron microscopy. Dilute coverage of magnetic beads allowed the red blood cells to be separated from solution in the presence of a magnetic field, eliminating the need for centrifugation. The magnetized glutaraldehyde-treated red blood cells were then functionalized with human immunoglobulin and used in flow cytometry for the determination of antihuman immunoglobulin. The results indicated that such magnetized glutaraldehyde-treated red blood cell may be used as carriers in flow cytometry. The approach may allow the preparation of carriers using other red blood cells.