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Thermo Hypersil™ BDS色谱柱

2017/11/07 华泰昕生物|HyperCyte 已读

要在各类应用中使用稳定通用的色谱柱,请选择 Thermo Scientific™ Hypersil™ BDS C18 HPLC 色谱柱。 这些经济型色谱柱具有较低硅烷醇活性,并为 QA/QC 实验室提供了出色的可靠性。 建立在著名的 Hypersil 硅胶基础之上的碱性去活硅胶是目前最流行的填料之一。

对于需要更强的色谱性能的更具挑战性的分离,请使用 Thermo Scientific™ Hypersil GOLD ™ 色谱柱作为替代。

Thermo Scientific Hypersil BDS色谱柱

碱性去活硅胶,更好的重现性和色谱峰对称性
 
 
Thermo Scientific Hypersil BDS 色谱柱自 1989 年发明以来,一直广受业界推崇,被认为是最耐用、重现性最好、最可信赖的色谱柱之一。Hypersil BDS 色谱柱以高度碱性去活硅胶为担体,采用封端技术屏蔽硅醇基, 具有以下优点:

  • 出色的重现性

  • 明显改善峰拖尾

  • 十分耐用,色谱柱使用寿命较长

  • 不论是碱性化合物还是酸性化合物,均呈现完美峰形。

为什么要用碱性去活硅胶和键合相?
 
使用共价键合硅胶固定相,可对多数化合物进行分离。HPLC 与液-液分配色谱相比,具有平衡速度快、分配性能更好的特点,因此,HPLC 作为现代分析手段被广泛应用。
 
但是,传统的共价键合硅胶固定相存在以下问题:
HPLC 中使用的建和硅胶固定相与分析物之间存在多种相互作用,这些相互作用一部分是键合相本身与分析物之间的作用,另一部分是键合时在硅胶表面残留的硅醇基与分析物之间的作用。
 
硅醇基的数目和酸性极大程度影响了它与分析物间相互作用的强弱。碱性硅胶的酸-碱相互作用主要是由硅醇基产生的,硅醇基还决定了硅胶填料的表面极性。硅醇基的种类和酸性在很大程度上影响了 HPLC 的分离度和分析物的色谱峰形。

残余硅醇基与化合物的相互作用会使酸性和碱性化合物的色谱峰产生拖尾。早期使用的 HPLC 硅胶填料,硅醇基具有很强的酸性,因此色谱拖尾现象特别明显。

使用早期硅胶填料时,若要得到较好的色谱峰,通常需要在流动相中添加酸(醋酸)或碱(三乙胺),使其与硅醇基产生竞争性结合,从而改善色谱峰形。当使用了合适的流动相添加剂时,色谱峰形会得到明显改善。通常需要很高的添加剂浓度——多数情况下达到 1% (体积比)或更高,这样才能使所有残留硅醇基都与添加剂结合,而不影响分析物的色谱峰形。但是高浓度添加剂常常严重降低色谱柱寿命和方法重现性。
 
所以Hypersil BDS 是最早的碱性去活硅胶之一,具有以下优点:

减弱硅醇基相互作用
改善色谱峰拖尾
不需向流动相中加入添加剂
极佳的色谱峰对称性
延长色谱柱使用寿命
提高柱效(分析碱性、酸性、中性化合物)
 
Hypersil BDS重现性,批次间测试流程,多种固定相:
 
Hypersil BDS 色谱柱是根据最高标准生产的,并进过严苛的质量控制。硅胶担体和色谱柱的生产流程均完全遵守 ISO9001:2000 标准。在进行键合前,BDS 硅胶必须通过 30 余项物理特性和色谱性能测试。在键合后,所有的 BDS C18 填料都经过色谱性能测试以及碳载量测试——该测试在封端前和封端后各进行一次。
 
Hypersil BDS 色谱柱有 4 种键合相供选择。所有 Hypersil BDS 色谱柱,均使用碱性去活工艺处理硅胶担体,再经封端处理屏蔽硅醇基,即使是分析碱性化合物,也极大程度改善了峰拖尾。每支 Hypersil BDS 色谱柱都带有《质量认证书》

 

货号 描述
28102-012101 10x2.1mm 2.4um BDS C18
28102-014001 10x4mm 2.4um BDS C18
28102-032130 30x2.1mm 2.4 um BDS C18
28102-034630 30x4.6mm 2.4 um BDS C18
28102-052130 50x2.1mm 2.4 um BDS C18
28102-054630 50x4.6mm 2.4 um BDS C18
28102-102130 100x2.1mm 2.4 um BDS C18
28102-104630 100x4.6mm 2.4 um BDS C18
28102-152130 150x2.1mm 2.4 um BDS C18
28102-154630 150x4.6mm 2.4 um BDS C18
28103-011001 10x1mm 3um Hypersil™ BDS C18 保护柱柱芯
28103-012101 10x2.1mm 3um Hypersil BDS C18 保护柱柱芯
28103-012106 10x2.1mm 3u BDS C18 Javelin 直连式保护柱
28103-013001 10x3mm 3um Hypersil BDS C18 保护柱柱芯 
28103-014001 10x4mm 3um Hypersil BDS C18 保护柱柱芯 
28103-014006 10x4mm 3u BDS C18 Javelin 直连式保护柱 
28103-022106 20x2.1mm 3u BDS C18 Javelin 直连式保护柱
28103-023006 20x3mm 3u BDS C18 Javelin 直连式保护柱
28103-032130 30x2.1mm 3um Hypersil BDS C18
28103-033030 30x3mm 3um Hypersil BDS C18
28103-034030 30x4mm 3um Hypersil BDS C18
28103-034630 30x4.6mm 3um Hypersil BDS C18
28103-051030 50x1mm 3u BDS C18
28103-052130 50x2.1mm 3um Hypersil BDS C18
28103-053030 50x3mm 3um Hypersil BDS C18
28103-054030 50x4mm 3um Hypersil BDS C18
28103-054630 50x4.6mm 3um Hypersil BDS C18
28103-074630 75x4.6mm 3u BDS C18
28103-101030 100x1mm 3u Hypersil BDS C18
28103-102130 100x2.1mm 3um Hypersil Hypersil BDS C18
28103-103030 100x3mm 3um Hypersil Hypersil BDS C18
28103-104030 100x4mm 3um Hypersil Hypersil BDS C18
28103-104630 100x4.6mm 3um Hypersil Hypersil BDS C18
28103-122130 125x2.1mm 3u BDS C18
28103-123030 125x3mm 3u BDS C18
28103-124030 125x4mm 3u BDS C18
28103-124630 125x4.6mm 3u BDS C18
28103-151030 150x1mm 3u BDS C18
28103-152130 150x2.1mm 3um Hypersil Hypersil BDS C18
28103-153030 150x3mm 3um Hypersil Hypersil BDS C18
28103-154030 150x4mm 3um Hypersil Hypersil BDS C18
28103-154630 150x4.6mm 3um Hypersil Hypersil BDS C18
28103-204630 200x4.6mm 3u BDS C18
28103-252130 250x2.1mm 3u Hypersil BDS C18
28103-253030 250x3mm 3u Hypersil BDS C18
28103-254030 250x4mm 3u Hypersil BDS C18
28103-254630 250x4.6mm 3u Hypersil BDS C18
28105-011001 10x1mm 5um Hypersil Hypersil BDS C18 保护柱柱芯 
28105-011006 10x1mm, 5u BDS C18, Javelin 直连式保护柱 
28105-012101 10x2.1mm 5um Hypersil Hypersil BDS C18 保护柱柱芯 
28105-012106 10x2.1mm 5u BDS C18 Javelin 直连式保护柱  
28105-013001 10x3mm 5um Hypersil Hypersil BDS C18 保护柱柱芯 
28105-013006 10x3mm 5u BDS C18 Javelin 直连式保护柱 
28105-014001 10x4mm 5um Hypersil Hypersil BDS C18 保护柱柱芯
28105-014006 10x4mm 5u BDS C18 Javelin 直连式保护柱  
28105-022106 20x2.1mm 5u BDS C18 Javelin 直连式保护柱 
28105-023006 20x3mm 5u BDS C18 Javelin 直连式保护柱 
28105-024006 20x4mm 5u BDS C18 Javelin 直连式保护柱 
28105-034630 30x4.6mm 5u Hypersil BDS C18
28105-052130 50x2.1mm 5um Hypersil Hypersil BDS C18
28105-053030 50x3mm 5um Hypersil Hypersil BDS C18
28105-054030 50x4mm 5um Hypersil Hypersil BDS C18
28105-054630 50x4.6mm 5um Hypersil Hypersil BDS C18
28105-059070 50x10mm 5u BDS C18 制备柱
28105-074630 75x4.6mm 5u Hypersil BDS C18
28105-102130 100x2.1mm 5um Hypersil Hypersil BDS C18
28105-103030 100x3mm 5um Hypersil Hypersil BDS C18
28105-104030 100x4mm 5um Hypersil Hypersil BDS C18
28105-104630 100x4.6mm 5um Hypersil Hypersil BDS C18
28105-122130 125x2.1mm 5um Hypersil Hypersil BDS C18
28105-123030 125x3mm 5um Hypersil Hypersil BDS C18
28105-124030 125x4mm 5um Hypersil Hypersil BDS C18
28105-124630 125x4.6mm 5um Hypersil Hypersil BDS C18
28105-152130 150x2.1mm 5um Hypersil Hypersil BDS C18
28105-153030 150x3mm 5um Hypersil Hypersil BDS C18
28105-154030 150x4mm 5um Hypersil Hypersil BDS C18
28105-154630 150x4.6mm 5um Hypersil Hypersil BDS C18
28105-159270 150x21.2mm 5u Hypersil BDS C18 制备柱
28105-202130 200x2.1mm 5um Hypersil Hypersil BDS C18
28105-203030 200x3mm 5um Hypersil Hypersil BDS C18
28105-204030 200x4mm 5um Hypersil Hypersil BDS C18
28105-204630 200x4.6mm 5um Hypersil Hypersil BDS C18
28105-252130 250x2.1mm 5um Hypersil Hypersil BDS C18
28105-252168 250x2.1mm 5um BDS C18 PEEK柱
28105-253030 250x3mm 5um Hypersil Hypersil BDS C18
28105-254030 250x4mm 5um Hypersil Hypersil BDS C18
28105-254630 250x4.6mm 5um Hypersil Hypersil BDS C18
28105-259070 250x10mm 5um Hypersil BDS C18 制备柱
28105-259270 250x21.2mm 5um Hypersil BDS C18 制备柱
28105-304030 300x4mm 5um Hypersil BDS C18
28105-304630 300x4.6mm 5um Hypersil BDS C18
28202-012101 10x2.1mm 2.4um Hypersil BDS C8
28202-014001 10x4mm 2.4um Hypersil BDS C8
28202-032130 30x2.1mm 2.4 um Hypersil BDS C8
28202-034630 30x4.6mm 2.4 um Hypersil BDS C8
28202-052130 50x2.1mm 2.4 um Hypersil BDS C8
28202-054630 50x4.6mm 2.4 um Hypersil BDS C8
28202-102130 100x2.1mm 2.4 um Hypersil BDS C8
28202-104630 100x4.6mm 2.4 um Hypersil BDS C8
28202-152130 150x2.1mm 2.4 um Hypersil BDS C8
28202-154630 150x4.6mm 2.4 um Hypersil BDS C8
28203-012101 10x2.1mm, 3um Hypersil Hypersil BDS C8 保护柱柱芯
28203-013001 10x3mm, 3um Hypersil Hypersil BDS C8 保护柱柱芯
28203-014001 10x4mm, 3um Hypersil Hypersil BDS C8 保护柱柱芯 
28203-032130 30x2.1mm 3u Hypersil BDS C8
28203-033030 30x3mm 3u Hypersil BDS C8
28203-034630 30x4.6mm 3u Hypersil BDS C8
28203-052130 50x2.1mm 3um Hypersil Hypersil BDS C8
28203-053030 50x3mm 3um Hypersil Hypersil BDS C8
28203-054030 50x4mm 3um Hypersil Hypersil BDS C8
28203-054630 50x4.6mm 3um Hypersil Hypersil BDS C8
28203-074630 75x4.6mm 3u Hypersil BDS C8 column
28203-102130 100x2.1mm 3um Hypersil Hypersil BDS C8
28203-103030 100x3mm 3um Hypersil Hypersil BDS C8
28203-104030 100x4mm 3um Hypersil Hypersil BDS C8
28203-104630 100x4.6mm 3um Hypersil Hypersil BDS C8
28203-123030 125x3mm 3u Hypersil BDS C8
28203-124630 125x4.6mm 3u Hypersil BDS C8
28203-152130 150x2.1mm 3um Hypersil Hypersil BDS C8
28203-153030 150x3mm 3um Hypersil Hypersil BDS C8
28203-154030 150x4mm 3um Hypersil Hypersil BDS C8
28203-154630 150x4.6mm 3um Hypersil Hypersil BDS C8
28203-252130 250x2.1mm 3u Hypersil BDS C8
28203-254630 250x4.6mm 3u Hypersil BDS C8
28205-011001 10x1mm, 5um Hypersil Hypersil BDS C8 保护柱柱芯
28205-012101 10x2.1mm, 5um Hypersil Hypersil BDS C8 保护柱柱芯
28205-012106 10x2.1mm 5u BDS C8 Javelin 直连式保护柱  
28205-013001 10x3mm, 5um Hypersil Hypersil BDS C8 保护柱柱芯
28205-014001 10x4mm, 5um Hypersil Hypersil BDS C8 保护柱柱芯
28205-014006 10x4mm 5u BDS C8 Javelin 直连式保护柱 
28205-022106 20x2.1mm 5u BDS C8 Javelin 直连式保护柱  
28205-023006 20x3mm 5u BDS C8 Javelin 直连式保护柱 
28205-024006 20x4mm 5u BDS C8 Javelin 直连式保护柱 
28205-032130 30x2.1mm 5u Hypersil BDS C8
28205-034630 30x4.6mm 5u Hypersil BDS C8
28205-052130 50x2.1mm 5um Hypersil Hypersil BDS C8
28205-053030 50x3mm 5um Hypersil Hypersil BDS C8
28205-054030 50x4mm 5um Hypersil Hypersil BDS C8
28205-054630 50x4.6mm 5um Hypersil Hypersil BDS C8
28205-102130 100x2.1mm 5um Hypersil Hypersil BDS C8
28205-103030 100x3mm 5um Hypersil Hypersil BDS C8
28205-104030 100x4mm 5um Hypersil Hypersil BDS C8
28205-104630 100x4.6mm 5um Hypersil Hypersil BDS C8
28205-124030 125x4mm 5u Hypersil BDS C8
28205-124630 125x4.6mm 5u Hypersil BDS C8
28205-152130 150x2.1mm 5um Hypersil Hypersil BDS C8
28205-153030 150x3mm 5um Hypersil Hypersil BDS C8
28205-154030 150x4mm 5um Hypersil Hypersil BDS C8
28205-154630 150x4.6mm 5um Hypersil Hypersil BDS C8
28205-159070 150x10mm 5u Hypersil BDS C8 制备柱
28205-204630 200x4.6mm 5u Hypersil BDS C8
28205-252130 250x2.1mm 5um Hypersil Hypersil BDS C8
28205-253030 250x3mm 5um Hypersil Hypersil BDS C8
28205-254030 250x4mm 5um Hypersil Hypersil BDS C8
28205-254630 250x4.6mm 5um Hypersil Hypersil BDS C8
28205-259270 250x21.2mm 5u BDS C8 PEEK柱
28205-304030 300x4mm 5u Hypersil Hypersil BDS C8
28205-304630 300x4.6mm 5u Hypersil BDS C8
28802-052130 50x2.1mm 2.4 um Hypersil BDS CN
28802-054630 50x4.6mm 2.4 um Hypersil BDS CN
28802-102130 100x2.1mm 2.4 um Hypersil BDS CN
28802-152130 150x2.1mm 2.4 um Hypersil BDS CN
28803-012101 10x2.1mm, 3um Hypersil Hypersil BDS Cyano 保护柱柱芯
28803-013001 10x3mm, 3um Hypersil Hypersil BDS Cyano 保护柱柱芯 
28803-014001 10x4mm, 3um Hypersil Hypersil BDS Cyano 保护柱柱芯
28803-052130 50x2.1mm 3um Hypersil Hypersil BDS Cyano
28803-053030 50x3mm 3um Hypersil Hypersil BDS Cyano
28803-054030 50x4mm 3um Hypersil Hypersil BDS Cyano
28803-054630 50x4.6mm 3um Hypersil Hypersil BDS Cyano
28803-102130 100x2.1mm 3um Hypersil Hypersil BDS Cyano
28803-103030 100x3mm 3um Hypersil Hypersil BDS Cyano
28803-104030 100x4mm 3um Hypersil Hypersil BDS Cyano
28803-104630 100x4.6mm 3um Hypersil Hypersil BDS Cyano
28803-152130 150x2.1mm 3um Hypersil Hypersil BDS Cyano
28803-153030 150x3mm 3um Hypersil Hypersil BDS Cyano
28803-154030 150x4mm 3um Hypersil Hypersil BDS Cyano
28803-154630 150x4.6mm 3um Hypersil Hypersil BDS Cyano
28805-011001 10x1mm, 5u Hypersil BDS CPS,
28805-012101 10x2.1mm, 5um Hypersil Hypersil BDS Cyano 保护柱柱芯 
28805-013001 10x3mm, 5um Hypersil Hypersil BDS Cyano 保护柱柱芯 
28805-014001 10x4mm, 5um Hypersil Hypersil BDS Cyano 保护柱柱芯 
28805-014006 10x4mm 5u BDS CPS Javelin 直连式保护柱
28805-052130 50x2.1mm 5um Hypersil Hypersil BDS Cyano
28805-053030 50x3mm 5um Hypersil Hypersil BDS Cyano
28805-054030 50x4mm 5um Hypersil Hypersil BDS Cyano
28805-054630 50x4.6mm 5um Hypersil Hypersil BDS Cyano
28805-102130 100x2.1mm 5um Hypersil Hypersil BDS Cyano
28805-103030 100x3mm 5um Hypersil Hypersil BDS Cyano
28805-104030 100x4mm 5um Hypersil Hypersil BDS Cyano
28805-104630 100x4.6mm 5um Hypersil Hypersil BDS Cyano
28805-152130 150x2.1mm 5um Hypersil Hypersil BDS Cyano
28805-153030 150x3mm 5um Hypersil Hypersil BDS Cyano
28805-154030 150x4mm 5um Hypersil Hypersil BDS Cyano
28805-154630 150x4.6mm 5um Hypersil Hypersil BDS Cyano
28805-204630 200x4.6mm 5u Hypersil BDS CPS
28805-252130 250x2.1mm 5um Hypersil Hypersil BDS Cyano
28805-253030 250x3mm 5um Hypersil Hypersil BDS Cyano
28805-254030 250x4mm 5um Hypersil Hypersil BDS Cyano
28805-254630 250x4.6mm 5um Hypersil Hypersil BDS Cyano
28805-304030 300x4mm 5u Hypersil Hypersil BDS CPS
28805-304630 300x4.6mm 5u Hypersil BDS CPS
28902-052130 50x2.1mm 2.4um Hypersil BDS Phenyl
28902-054630 50x4.6mm 2.4um Hypersil BDS Phenyl
28902-102130 100x2.1mm 2.4u Hypersil BDS Phenyl
28902-154630 150x4.6mm 2.4u Hypersil BDS Phenyl
28903-012101 10x2.1mm, 3um Hypersil Hypersil BDS Phenyl 保护柱柱芯
28903-013001 10x3mm, 3um Hypersil Hypersil BDS Phenyl 保护柱柱芯 
28903-014001 10x4mm, 3um Hypersil Hypersil BDS Phenyl 保护柱柱芯 
28903-032130 30x2.1mm 3u Hypersil BDS Phenyl
28903-052130 50x2.1mm 3um Hypersil Hypersil BDS Phenyl
28903-053030 50x3mm 3um Hypersil Hypersil BDS Phenyl
28903-054030 50x4mm 3um Hypersil Hypersil BDS Phenyl
28903-054630 50x4.6mm 3um Hypersil Hypersil BDS Phenyl
28903-102130 100x2.1mm 3um Hypersil Hypersil BDS Phenyl
28903-103030 100x3mm 3um Hypersil Hypersil BDS Phenyl
28903-104030 100x4mm 3um Hypersil Hypersil BDS Phenyl
28903-104630 100x4.6mm 3um Hypersil Hypersil BDS Phenyl
28903-152130 150x2.1mm 3um Hypersil Hypersil BDS Phenyl
28903-153030 150x3mm 3um Hypersil Hypersil BDS Phenyl
28903-154030 150x4mm 3um Hypersil Hypersil BDS Phenyl
28903-154630 150x4.6mm 3um Hypersil Hypersil BDS Phenyl
28905-012101 10x2.1mm, 5um Hypersil Hypersil BDS Phenyl 保护柱柱芯
28905-013001 10x3mm, 5um Hypersil Hypersil BDS Phenyl 保护柱柱芯 
28905-014001 10x4mm, 5um Hypersil Hypersil BDS Phenyl 保护柱柱芯 
28905-022106 20x2.1mm 5u BDS Phenyl Javelin 直连式保护柱 
28905-052130 50x2.1mm 5um Hypersil Hypersil BDS Phenyl
28905-053030 50x3mm 5um Hypersil Hypersil BDS Phenyl
28905-054030 50x4mm 5um Hypersil Hypersil BDS Phenyl
28905-054630 50x4.6mm 5um Hypersil Hypersil BDS Phenyl
28905-102130 100x2.1mm 5um Hypersil Hypersil BDS Phenyl
28905-103030 100x3mm 5um Hypersil Hypersil BDS Phenyl
28905-104030 100x4mm 5um Hypersil Hypersil BDS Phenyl
28905-104630 100x4.6mm 5um Hypersil Hypersil BDS Phenyl
28905-152130 150x2.1mm 5um Hypersil Hypersil BDS Phenyl
28905-153030 150x3mm 5um Hypersil Hypersil BDS Phenyl
28905-154030 150x4mm 5um Hypersil Hypersil BDS Phenyl
28905-154630 150x4.6mm 5um Hypersil Hypersil BDS Phenyl
28905-159270 150x21.2mm 5u Hypersil BDS Phenyl 制备柱
28905-204630 200x4.6mm 5u Hypersil BDS Phenyl
28905-252130 250x2.1mm 5um Hypersil Hypersil BDS Phenyl
28905-253030 250x3mm 5um Hypersil Hypersil BDS Phenyl
28905-254030 250x4mm 5um Hypersil Hypersil BDS Phenyl
28905-254630 250x4.6mm 5um Hypersil Hypersil BDS Phenyl
28905-304030 300x4mm 5u Hypersil BDS Phenyl
28905-304630 300x4.6mm 5u Hypersil BDS Phenyl