Bangs Laboratories

功能化聚苯乙烯微球(羧基PC和氨基PA)

2019/06/27 116

华泰昕

Our carboxylate-modified polystyrene latex microspheres are suitable for the covalent immobilization of proteins, peptides, and nucleic acids. They are synthesized via emulsion polymerization, and are available in diameters up to ~20µm, with typical size CVs of 10%. Most products are supplied as 10% solids suspension (w/w) in de-ionized water with surfactant and sodium azide.


羧基化聚苯乙烯微球

EDAC-mediated coupling is often used for the covalent immobilization of amine-terminated proteins, DNA, or other molecules on carboxyl-functionalized PS and P(S/DVB) microspheres. Carboxyl polymer microspheres are available as ~10% solids (w/w) aqueous suspensions in these standard amounts: 0.5g, 1.0g, 1.5g, and 5.0g.

New to beads? See our PolyLink Protein Coupling Kit and TechNote 205, Covalent Coupling, for a protocol and tips on coating optimization and QC.

*Please note: If you are NOT ordering via website, please specify a lot number if multiple lots are available. 
Catalog NumberNominal Diameter

Specification Range

PC020010.025µm0.015 - 0.035µm
PC020020.050µm0.040 - 0.060µm
PC020030.070µm0.061 - 0.080µm
PC020040.100µm0.090 - 0.110µm
PC020050.125µm0.115 - 0.135µm
PC020060.150µm0.140 - 0.160µm
PC020070.175µm0.165 - 0.185µm
PC020080.200µm0.190-0.210µm
PC020090.300µm0.270-0.330µm
PC020100.350µm0.340 - 0.360µm
PC020110.400µm0.370 - 0.430µm
PC030010.500µm0.470 - 0.530µm
PC030020.600µm0.570 - 0.630µm
PC030030.800µm0.770 - 0.830µm
PC030040.900µm0.870 - 0.930µm
PC040011.0µm0.95 - 1.05µm
PC050012.00µm1.80 - 2.20µm
PC050022.50µm2.30 - 2.70µm
PC050033.00µm2.80 - 3.20µm
PC050044.00µm3.80 - 4.20µm
PC050054.50µm4.30 - 4.70µm
PC060015.00µm4.80 - 5.20µm
PC060025.50µm5.30 - 5.70µm
PC060036.00µm5.80 - 6.20µm
PC060047.00µm6.80 - 7.20µm
PC0700110.00µm9.50 - 10.50µm
PC0700215.00µm14.50µm - 15.50µm
PC0700320.00µm19.00µm - 21.00µm
PC08001>25.µm>25.00µm

参考文献

Park, MK, Briles, DE, Nahm, MH (2000) A Latex Bead-Based Flow Cytometric Immunoassay Capable of Simultaneous Typing of Multiple Pneumococcal Serotypes (Multibead Assay) Clin Vaccine Immunol v7(3) 486-489

Blanchette C, Lacayo CI, Fischer NO, Hwang M, Thelen MP (2012) Enhanced Cellulose Degradation Using Cellulase-Nanosphere Complexes. PLoS ONE 7(8): e42116

Rodrigues OR, & Monard S, (2016) A rapid method to verify single-cell deposition setup for cell sorters. Cytometry 89: 594–600

Borque L, Bellod L, Rus A, Seco L, Galisteo-Gonzàles F (2000) Development and Validation of an Automated and Ultrasensitive Immunoturbidimetric Assay for C-Reactive Protein. Clin Chem 46(11) 1839-1842

Perez-Amodio S, Holownia P, Davey C, Price C (2001) Effects of the Ionic Environment, Charge, and Particle Surface Chemistry for Enhancing a Latex Homogeneous Immunoassay of C-Reactive Protein. Anal. Chem.73 (14) 3417–3425

Lucas L, Han J, Yoon J (2006) Using highly carboxylated microspheres to simplify immunoassays and enhance diffusional mixing in a microfluidic device. Colloids and Surf. B: Biointerfaces 49(2) 106-111

Fu L, Kang C, Zhang P, Kang X, Li X, Wang H, …Qiu Y (2015) A modified quick PETIA for detecting anti-CCP antibodies in human serum, Anal Methods Issue 7

Deegan O, Walshe K, Kavanagh K, Doyle S. (2003) Quantitative detection of C-reactive protein using phosphocholine-labelled enzyme or microspheres. Anal. Biochem. 312 p175–181 (turbidimetric assay, 0.21µm PS-COOH)

Koskinen JM, Soukka JM, Meltola NJ, & Koskinen JO. (2018). Microbial identification from faces and urine in one step by two-photon excitation assay technique. Journal of immunological methods, 460, 113-118.(Cat. PC05003, 3.2µm PS-COOH microspheres, coated with anti-Legionella or anti-adenovirus Ab in ArcDia’s TPX assay.)

Nanavaty N, Lin L, Hinckley SH, & Kuret J. (2017) Detection and quantification methods for fibrillar products of in vitro tau aggregation assays. In Tau Protein (pp. 101-111). Humana Press, New York, NY.(Anionic PS-COOH, 40-90nm as tau aggregation inducers)

氨基聚苯乙烯微球

Amine-functionalized microspheres offer further options for coupling of biomolecules. Glutaraldehyde is commonly used for the immobilization of amine-containing compounds, and specialized linkers are available for targeted coupling of thiol or carbohydrate moieties. See TechNote 205, Covalent Coupling. Unless otherwise noted amine polymer microspheres are available as ~10% solids (w/w) aqueous suspensions in these standard amounts: 0.5g, 1.0g, 1.5g, and 5.0g. For microspheres larger than 1.00µm, please call for availability.

*Please note: If you are NOT ordering via website, please specify a lot number if multiple lots are available. 
Catalog NumberNominal Diameter

Specification Range

Product Data Sheet
PA020010.200µm0.190 - 0.210µmPDS 732.pdf
PA030010.500µm0.470 - 0.530µmPDS 732.pdf
PA030020.750µm0.720 - 0.780µmPDS 732.pdf
PA040011.0µm0.95 - 1.05µmPDS 732.pdf

参考文献

Schleicher KD, Dettmer SL, Kapinos LE, Pagliara S, Keyser UF, Jeney S, LimRoderick YH (2014) Selective transport control on molecular velcro made from intrinsically disordered proteins. Nat Nano 9, 525-530

Bandura DR, Baranov VI, Ornatsky OI, Antonov A, Kinach R, Lou X… Tanner S.(2009) Mass Cytometry: Technique for Real Time Single Cell Multitarget Immunoassay Based on Inductively Coupled Plasma Time-of-Flight Mass Spectrometry. Anal Chem 81,6813–6822